摘要
本研究观察了心肌肥厚大鼠左室组织中肌醇磷脂途径特征。对大鼠行腹主动脉部分缩窄术制作心肌肥厚模型 ,术后 10d处死动物测全心重 体重比值 ,以免疫印迹法测左室组织Gαq 11和PLC β3 蛋白含量 ,以放免法测左室组织 1,4,5 三磷酸肌醇 (IP3 )含量。结果显示术后 10d时腹主动脉部分缩窄 (CA)组大鼠全心重 体重比值明显高于假手术 (SO)组 (P <0 0 1) ,二组大鼠左室组织Gαq 11和PLC β3 蛋白含量无显著差异 ,CA组左室组织IP3 浓度明显高于SO组 (P <0 0 5 )。
We identified the phosphoinositide pathway in left ventricular tissue of rats with cardiac hypertrophy. Cardiac hypertrophy was induced in male Sprague-Dawley rats with coarctation of abdominal aorta (CA), whole heart weight/body weight ratio was recorded after 10 days. Gαq/11 and PLC-β 3 protein in left ventricle were detected by immunoblot analysis, concentration of inositol 1,4,5- trisphosphate (IP 3) was measured by radioimmunoassay. At 10th day, heart weight/ body weight ratio of CA rats was higher than that of sham-operated(SO) animals (P<0.01), contents of Gαq/11 and PLC-β 3 did not show difference in left ventricle between two groups, the level of IP 3 was significantly increased in the left ventricle of CA rats after 10 days of stenosis (P<0.05). These results suggested that phosphoinositide pathway played a role in the pathogenesis of cardiac hypertrophy induced by pressure overload.
出处
《基础医学与临床》
CSCD
北大核心
2004年第1期37-39,共3页
Basic and Clinical Medicine
基金
国家自然科学基金 ( 3 9670 3 14 )
