实验感染肝片吸虫山羊的病理生理学 Ⅱ.血清NO和花生四烯酸代谢物的变化

Pathophysiology of Fasciola hepatica Infection in GoatsⅡ.Kinetic Changes of NO,6-keto-PGF_(1α) and TXB_2

选择 9只 6～ 10月龄健康雄性白山羊 ,经粪便检查和 Dot- EL ISA检测 ,确认无肝片吸虫感染 ,随机分成感染组(n=5 )和对照组 (n=4 ) ,试验组每只一次性口服接种 15 0个肝片吸虫囊蚴 ,感染前 (0周 )至感染后 2 3周每周从颈静脉采集血液 1次 ,分离血清 ,测定一氧化氮含量和花生四烯酸代谢物 6 -酮 -前列腺素 F1α(6 - keto- PGF1α)和血栓素 B2(TXB2 )的变化。结果表明 ,肝片吸虫感染后第 6～ 2 1周试验组血清中的 NO一直高于对照组 ,并在第 7、10周时显著地高于对照组 ;血浆中 TXB2 水平变化较为明显 ,在 1～ 3周与对照组相比无显著差异 ,从第 5周开始到试验结束 ,试验组血浆中 TXB2 水平均显著或极显著地高于对照组 ;在整个试验期间 ,血浆中 6 - keto- PGF1α水平变化不显著。这一结果提示 ,NO及花生四烯酸代谢物可能参与山羊肝片吸虫病的发生发展过程。

Nine healthy male no fascioliasis goats confirmed by fecal examination and Dot-ELISA were chosen and randomly divided into infection group(n=5) and control group(n=4).Each goat of infection group was orally dosed once 150 Fasciola hepatica metacercariae.Blood sample was collected by jugular vein per week to determine nitric oxide(NO),6-keto-PGF_(1α),thromboxane B_2(TXB_2).The results showed that the level of NO increased after infection.The level change of TXB_2 was significant.At 5-23 weeks postinfection,TXB_2 of experiment group was obvious higher than that of control group.There was no obvious change of 6-keto-PGF_(1α) during experiment.The results suggested that NO and arachidonic acid may participate in the pathogenesis of fascioliasis.