摘要
目的:探讨HPV16在宫颈鳞癌发生发展中的作用及其与c-myc、p53表达产物之间的关系。方法:采用原位杂交和免疫组化方法检测了18例慢性宫颈炎、28例CIN、4例宫颈浸润性腺鳞癌和55例宫颈浸润性鳞癌HPV16 E6 DNA及其蛋白和c-myc、p53蛋白的表达情况。结果:浸润性宫颈癌HPV16 E6 DNA及其蛋白、p53蛋白阳性率明显高于慢性宫颈炎组,c-myc蛋白阳性率则明显低于慢性宫颈炎组,p53蛋白阳性率与HPV16 E6 DNA的检出率之间无负相关关系。结论:宫颈鳞癌的发生发展可能与HPV16 E6 DNA的检出及c-myc、p53蛋白的表达异常相关。
Objective:To study the role of HPV16infection in the carcinogenesis of cervical squa-mous carcinoma and the relationship between HPV16infection and the expression of c - myc,p53protein in cervical squamous carcinoma.Methods:In Situ hybridization and immunohistochemical method were used to detect HPV16E6DNA,E6protein,c - myc and p53protein in18cases of chronic cervicitis,28cases of cervical intraepithelial neoplasia and59cases of invasive cervical carcinoma.Results:A higher detection rate of HPV16E6DNA,E6protein and p53protein were found in invasive cervical carcinoma which c - myc positive expression rate was significantly lower than chronic cervicitis.Conclusion:Carcinogenesis of cervi-cal squamous carcinoma may be related to the presence of HPV16E6DNA and the abnormal expression of c - myc,p53protein.
出处
《肿瘤研究与临床》
CAS
2004年第1期7-10,共4页
Cancer Research and Clinic
