SARS-CoV S蛋白基因的克隆及其与VSV-G融合表达载体的构建

Cloning of the gene of SARS-CoV S protein and construction of the fusion expression vector of S protein with VSV-G

为了解重症急性呼吸综合征冠状病毒(SARS-CoV)表面S蛋白的受体结合功能域及其在宿主细胞上的作用受体,应用PCR技术从SARS-CoVcDNA中克隆到S蛋白的全长基因,并构建了S蛋白与疱疹性口腔炎病毒胞膜蛋白(VSV-G)融合表达载体pVSV-G'-SG,进而为制备含有SARS-CoVS蛋白膜外区的逆转录病毒假毒粒奠定了实验基础。

The tropism or host range could be altered by changing the viral envelope protein.In order to find the recep-tors in the host cells and invest the receptor binding domain of the severe acute respiratory syndrome coronavirus(SARS-CoV)spiking(S)protein,the full gene of S protein was cloned from the complete cDNA of SARS-CoV,and the fusion ex-pression vector pVSV-G'-SG was constructed with the extracellular domain of S protein and transmembrane region and cy-toplasmic tail of vesicular stomatitis virus(VSV)G glycoprotein by PCR method.This work is the first step to generate pseudotyped virus with S/VSV-G chimeric envelope.