应用PCR/RFLP技术对广西禽白血病病毒的检测与分型研究

Detection and sub-grouping of ALVs in Guangxi chickens by PCR/RFLP technique

对2000～2003年间从广西的南宁、玉林、桂林、钦州、柳州、梧州等地采集的有肿瘤/可疑肿瘤的250份鸡病例进行肿瘤病的检测。结果发现,外源性禽白血病阳性的有32份,阳性率为12.8%。其中,同时为马立克氏病阳性的有30份,两者共感染率高达93.75%(30/32);根据禽白血病病毒囊膜糖蛋白gp85基因的限制性片段长度多态性分析,对其中20份外源性禽白血病病毒进行分型鉴定,结果证实全部属于A亚群。

250 birds with tumors collected from Nanning, Yulin, Guilin, Qinzhou, Liuzhou, Wuzhou during the years of 2000 to 2003 were detected for exogenous avian leukosis virus (ALV) and Marek's disease virus (MDV-1) by a tumors detection kit developed in the laboratory. The results show that 32 (12.8 %) birds were positive of exogenous ALVs, in which 30 (93.75 %) birds were also positive of virulent MDV-1. 20 samples of the exogenous ALVs were randomly used for the sub-grouping by RFLP(restriction fragment length polymorphism)analysis of the glycoprotein gene gp85 of the viral genomes, and the results show that they are all belong to subgroup A.