钙和硼对蓝猪耳花粉萌发及花粉管生长的影响

Effects of Calcium and Boron on Pollen Germination and Pollen Tube Growth of Torenia fournieri

研究了钙(Ca2+)和硼(H3BO3)对蓝猪耳花粉萌发和花粉管生长的影响。结果表明:(1)在一定范围内Ca2+几乎不影响花粉萌发频率,而主要影响花粉萌发速度和花粉管生长速度;低Ca2+不利于花粉管生长,而高Ca2+抑制花粉萌发速度和花粉管生长;在稍高于最适Ca2+浓度的条件下,花粉管生长早期呈现波浪形。(2)硼明显影响花粉萌发频率及花粉管形态;花粉管生长必需硼,但不同浓度的硼对花粉管生长速度影响不明显;在高浓度硼条件下,较长时间内花粉管均呈现出波浪形。(3)Cooled-CCD动态跟踪观察进一步证实Ca2+影响花粉管生长速度,而硼则不明显。

The effects of calcium (Ca^(2+)) and boron (H_3BO_3) on pollen germination and pollen tube growth of Torenia fournieri Lind.were investigated in this paper.The main results were following:(1) Ca^(2+) affected the speed of pollen germination and growth dramatically,but had no influence on the frequency of pollen germination.When the concentration of Ca^(2+) was very low or high,the pollen tubes grew poorly.When the concentration of Ca^(2+) was higher than proper concentration,the growth of pollen tubes was inhibited,and the pollen tubes became snaky in their early stages of growth.(2) Boron affected the frequency of pollen germination and the morphology of the pollen tube greatly,but different concentration of boron had no obvious effect on the growth speed of pollen tubes.When the concentration of boron was adjusted to higher than the proper concentration,the growth of pollen tubes became snaky for a long time.(3) Using cooled-CCD,serial photography further proves that it is the concentration of Ca^(2+) not that of H_3BO_3 that plays an important role for the growth speed of pollen tubes.