桃不同类群的遗传多样性及其遗传结构的RAPD分析

Genetic Diversity and Genetic Structure of Different Groups in Prunus persica Revealed by RAPD Analysis

采用RAPD技术,利用从200个随机引物筛选的22个十碱基引物对桃10个类群180个样品的DNA进行扩增,得到180个位点。通过对所得的数据统计分析,在类群的遗传多样度上表现为:黄肉桃类>蜜桃类>蟠桃类>红叶桃类>硬肉桃类>碧桃类>水蜜桃类>油桃类>寿星桃类>垂枝桃类。在180个位点中检验出多样度范围在0.4以上的位点分布在桃总群体中的有37个,而在桃类群间的则有13个。聚类分析的结果是:桃食用栽培品种的类群聚为一组;其它非食用桃类群各为一组。对桃类群的遗传多样性及其结构分析,为桃的品种资源保存和利用提供分子生物学依据。

DNAs of 180 accessions belonged to 10 groups in Prunus persica were amplified with 22 ten-base primers selected from 200 arbitrary primers using RAPD technology.180 loci were observed and recorded.With statistical analyses of the data from this experiment,genetic diversity of the groups expressed as follow:yellow peach group>mitao group>flat peach group>red-leaf peach group>crisp peach group>bitao group>honey peach group>nectarine group>shouxingtao group>weeping peach group.Of 180 loci,37 had higher genetic diversity in the total individuals,while 13 exsited between groups.Groups clustered by UPGMA modified from NEIGHBOR procedure of PHYLIP version 3.5,edible peach ones of which were combined as a set,while the others belonged to each set separately.Through analyses of genetic diversity and genetic cons-(truction),they can provide biomolecular evidence for conservation and utility of germplasm of P.(persica) .