大豆胞囊线虫胞囊内寄生真菌研究

THE RESEARCH ON THE CYST ENTOPARASITIC FUNGI OF SOYBEAN CYST NEMATIDE

从辽宁沈阳、黑龙江伊春、山东青岛、山西汾阳等地采集的大豆胞囊线虫土样 ,经分离获得定殖于胞囊上的真菌菌株 12 8株。通过测定菌株对胞囊的寄生率 ,筛选出 6株对大豆胞囊线虫有强寄生能力的胞囊内寄生真菌 ,经鉴定为Aspergillusniger ,Paecilomyceslilacinus ,Fusariumsemitectum ,Verticilliumchlamydosporium ,Verticilliumsp .,Acremoniumsp .,其中Aspergillusniger ,Fusariumsemitectum为国内首次报道定殖于大豆胞囊线虫胞囊上的真菌。通过 6株胞囊内寄生真菌对大豆胞囊线虫二龄幼虫毒性作用的研究 ,结果表明Verticilliumchlamydosporium和Verticilliumsp .两株菌的发酵液对大豆胞囊线虫二龄幼虫有较强毒杀作用。

One hunderd and twenty-eight fungi strains were isolated from the cysts of Heterodera glycines of the soybean rhizosphere soil in Liaoning, Hilongjiang, Shanxi, Shandong provinces. Through determining the parasitic ratio, six strains identified as Aspergillus niger, Paecilomyces lilacinus, Fusarium semitectum, Verticillium chlamydosporium, Verticillium sp. and Acremonium sp. were strong entoparasitic fungi of the cyst of H. glycines. Aspergillus niger and Fusarium semitectum isolated from the cyst were first reported in China. When the culture filtrates of six fungal strains were tested for their toxic effect to Second-stage Juveniles (J2) of H. glycines, the two strains, Verticillium chlamydosporium and Verticillium sp., had lethal effects on J2 of H. glycines, and their death ratios reached to 50.1% and 50.0%.