桃矮化砧木的组织培养及植株再生

Tissue culture and plantlet regeneration of dwarf Prunus persica rootstock

以豫农矮砧1号的茎段和茎尖为外植体,进行组织培养试验.结果表明,茎段芽诱导的适宜培养基为MS+6＿BA1 0mg·L-1+IBA0 1mg·L-1+GA31 0mg·L-1,茎尖芽诱导的适宜培养基为MS+6＿BA0 2mg·L-1+IBA0 01mg·L-1+GA31 0mg·L-1,幼芽形成根的最佳培养基为1/2MS+IBA1 0mg·L-1+IAA1 5mg·L-1,获得了豫农矮砧1号的再生植株,该研究结果为豫农矮砧1号的快速繁育奠定了基础.

Two explants of dwarf Yunong rootstock No.1 including stem segments and tips were cultured in vitro.The results showed that the suitable media for the bud induction from stem segments and tips were MS+6_BA 1.0 mg·L^(-1)+IBA 0.1 mg·L^(-1)+GA_3 1.0 mg·L^(-1) and MS+6_BA 0.2 mg·L^(-1)+IBA 0.01 mg·L^(-1)+GA_3 1.0 mg·L^(-1),and 1/2MS(+IBA) 1.0 mg·L^(-1)+IAA 1.5 mg·L^(-1) was the optimum medium of the rooting from shootings, the regenerated plantlets wene obtained in this experiment. These results might provide references for the quick propagation of dwarf Yunong rootstock No.1.