硅-玻璃聚合酶链式反应微芯片对β-葡糖苷酸酶基因的扩增

A Silicon-glass Micro-polymerase Chain Reaction Chip for GUS Gene Amplification

聚合酶链式反应 (PCR)微芯片是基于微机电系统 (MEMS)制作 ,在微芯片上进行PCR反应 ,实现生物样品扩增的一项新技术 .介绍了硅 玻璃PCR微芯片的设计和制作、微反应腔的清洗和表面处理、借助外置温度控制系统进行PCR扩增反应以及扩增产物在琼脂糖凝胶电泳下的检测分析 ,实现了对 β 葡糖苷酸酶 (GUS)基因的有效扩增 ,扩增时间由原来的 90min缩短到现在的 37min .

A micro polymerase chain reaction chip fabricated by MEMS technology, was successfully used to amplify the DNA molecule of GUS gene. Kinds of micro chamber PCR chip were designed and fabricated. The surface of the chamber was washed and prepared using a special method. Sample amplification was performed with thermal cycling system. The GUS gene amplified in chip was analyzed by slab gel electrophoresis with separation of DNA markers in parallel. The polymerase chain reaction was successfully performed in chip. The rapid PCR was completed in 37 min for 24 cycles. The thermal cycle time in chip was reduced to 1/2 with a commercial PCR instrument.