用激光共聚焦显微术检测哺乳动物卵母细胞中纺锤体相关蛋白的方法(英文)

Methodology for Detection of Spindle-associated Proteins by Confocal Microsco py in Mammalian Oocytes

哺乳动物卵母细胞是发育生物学和细胞生物学的重要研究对象 .激光共聚焦显微术是检测卵母细胞中纺锤体相关蛋白的有用技术 ,但是针对哺乳动物卵母细胞的特点需要做许多调整以得到理想结果 .其中 ,卵母细胞的准备、固定、透膜、脂类抽提、抗体孵育步骤十分关键 .另外 ,不同物种的卵母细胞也有差异 。

The mammalian oocytes are important materials for s tudy of meiotic cell cycle in developmental biology and cell biology. Confocal microscopy is a powerful technique in detecting the spindle associated proteins in oocytes. However, in order to obtain the ideal results, some technological modifications should be made according to the characteristics of mammalian oocytes. Among them, the oocyte preparation, fixation, membrane permeabilization, fat extraction, and antibody incubation are the key steps. Furthermore, the differences among oocytes from various mammalian species should be considered in the actual manipulation of confocal microscopy.