摘要
应用农杆菌EHA101,携带抗卡那霉素基因(氨基葡糖磷酸转移酶)和GUS基因(葡糖苷酸酶),对苹果品种‘Greensleeves’作基因导入获得成功,提出了应用农杆菌与苹果叶片外植体共培养并经卡那霉素筛选后,早期检测出已导入外源基因的愈伤组织。然后,通过愈伤组织增殖和应用TD7诱导获得不定芽,从而获得转基因的苹果试管苗。这一技术将有利于提高获得转基因植株的成功率。
The gene transformation of plant cells by genetically engineered strains of Agrobacterium tumefaciens has been studied extensively. This report describes the process of obtaining transgenic apple plants. Using A. tumefaciens strain EHA 101 carrying binary vector pCGN700l to inoculate leaf strips of apple cv. Greensleeves we have got transformed callus. After propagation of the callus on modified N6 medium added l0mg/L BA and 3mg/L NAA, the calli were induced to form adventitious shoots by using TDZ. By GUS assay almost all shoots are transgenic but one of them. Perhaps the sample of it was too small to examine. Therefore it would be better that the callus selected by kanamy-cin was propagated and induced to form shoots. In this way will obtain more and more transgenic shoots, and increase the efficiency of gene transformation.
出处
《园艺学报》
CAS
CSCD
北大核心
1992年第2期101-104,共4页
Acta Horticulturae Sinica
关键词
苹果
农杆菌
基因转移
葡糖苷酸酶
Apple
Agrobacterium
Gene tranfer
β-glucuronidase
