植物中硝态氮、氨态氮、总氮测定方法的比较研究

Study on the Method for the Determination of Nitric Nitrogen, Ammoniacal Nitrogen and Total Nitrogen in Plant

植物样品经硫酸 +过氧化氢凯氏法消煮后 ,分别用直接吸光光度法、靛酚蓝比色法、过硫酸钾氧化吸光光度法获得硝态氮 (包括亚硝态氮 )、氨态氮、总氮的含量。通过样品测试数据的比较 ,证明分别测得的氨态氮、硝态氮含量之和与所测总氮结果一致。

This paper reports the determination of three nitrogen states in plants: nitric nitrogen, ammoniacal nitrogen and total nitrogen. The plant sample was digested by the Kjeldathl method with H 2SO 4+H 2O 2. The solution of the sample was determined by three methods: absorption photometric method, indigotic colorimetry, and UV absorption photometric method after K 2S 2O 8 oxidation. By comparing the results, the relationship among the three N states is that the total N= NH + 4+NO - 3. Suitable methods were found for the determination of the elements, thus providing a scientific basis for the selection of suitable methods for chemical element analysis in plants.