农药甲胺磷人工抗原的光谱鉴定研究

Study on Spectral Identification of Methamidophos Artificial Antigen

对农药甲胺磷人工抗原BSAM的光谱进行了鉴定 ,鉴定结果表明 :人工抗原BSAM的紫外光谱图与原载体和半抗原的紫外光谱相比均发生变化 ;人工抗原BSAM的红外光谱图上具有与甲胺磷分子相似的P—O—C和PO特征吸收峰 ;人工抗原BSAM具有与标准甲胺磷一致的3 1 PNMR化学位移峰 ,磷钼蓝分光光度法测定人工抗原BSAM中的磷浓度后计算出人工抗原BSAM的偶联率为 1 1。4种光谱方法测定结果综合表明甲胺磷人工抗原BSAM合成成功。

In this paper, spectral identification of methamidophos artificial antigen was reported. The results showed that UV absorbance spectrum of artificial antigens of BSAM changed compared with carrier BSA and hapten Met. The absorbance peaks which are similar to P—O—C and P[ZJLX,Y]O moiety in IR spectrum of Met molecule appeared in the IR spectrum of the artificial antigens of BSAM. The chemical shift of the artificial antigens of BSAM is the same as that of standard Met in 31 P NMR spectrum. Phosphor concentration of the artificial antigens of BSAM was determined by phosphor molybdate blue spectrophotometry, and then conjugating ratio was calculated, to be 11. The results obtained with the above four spectrum methods showed that the artificial antigens of BSAM was synthesized successfully.