苦丁茶中熊果酸的提取工艺

Extraction of ursolic acid from Ilex Kudingcha by alcohol reflux

在苦丁茶中提取熊果酸的过程中,采用正交实验研究了乙醇体积分数、液固比、提取时间、提取温度、提取次数等对熊果酸浸出率的影响。正交实验的方差分析结果表明:对于熊果酸的浸出率,提取时间的影响最大,乙醇浓度和固液比的影响次之;最佳回流提取工艺条件为:在85℃水浴中,以90%(体积分数)乙醇水溶液为提取液,液固比为14∶1,提取2次,每次提取2.5h时,熊果酸的浸出率可达到1.44%。

The process of ursolic acid extraction from Ilex Kudingcha C. J. Tseng was studied. The optimal conditions were obtained by studying the effects of ethanol concentration, feedstock ratio, extraction time, extraction times on extraction rate of ursolic acid. The results of orthogonal experiment and variance analysis indicate that extraction time affects leaching rate of ursolic acid remarkably, ethanol concentration and feedstock ratio have finite effect. Maintainning react temperature at 85 ℃, leaching rate of ursolic acid is up to 1.44% by using 90% alcohol reflux extraction from Ilex Kudingcha C.J.Tseng twice each for 2.5 h at 1∶14 of Ilex Kudingcha C.J.Tseng mass to solvent volume.