青藤碱治疗大鼠实验性自身免疫性脑脊髓炎的研究

The Treatment of Experimental Autoimmune Encephalomyelitis in Rats by Sinomenine

目的:探讨盐酸青藤碱对大鼠实验性自身免疫性脑脊髓炎(EAE)的治疗作用。方法:对EAE大鼠动物模型给予3种不同剂量的青藤碱,并以甲氨蝶呤作为对照,观察青藤碱对EAE动物模型发病程度、脑和脊髓的病灶数目及血清肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、IL-10的影响。结果:青藤碱减轻EAE动物发病程度、减少脑和脊髓的病灶数目,降低血清TNF-α、IFN-γ水平,但对血清IL-10水平无明显影响。结论:青藤碱具有治疗EAE动物模型的作用,其机制可能与抑制TNF-α、IFN-γ为主的免疫应答有关。

Objective: To investigate the therapeutic efficacy and mechanism of Sinomenine on the treatment of experimental autoimmune encephalomyelitis (EAE) in rats. Methods: Sinomenine was administered in three different doses to female Wistar rats, which had been immunized with both brain and spinal cord extracts together with complete Freunds adjuvant. The severity of the disease was score d on a scale 0-6 according to the signs and symptoms. Histological examinations were performed in the sections of brain and spinal cord sections. The serum TNF -α,IFN-γand IL-10 were examined by ELISA. Results: Sinomenine treatment in three different doses all had a significantly protective action on the disease model. The protective role of Sinomenine was associated with significant reducti on in the numbers of inflammatory focus in the brain and spinal cord, and decrea se of the levels of serum TNF-α, and IFN-γ. Conclusion: The anti-inflammato ry role of Sinomenine may be a potential candidate for use in treating patients with multiple sclerosis. [