肾细胞癌中p27表达与肿瘤细胞增殖的关系研究(英文)

Study of expression of p27 in renal cell carcinoma and its relationship with tumor cell proliferation

目的 探讨肾细胞癌 (RCC)中p2 7的表达与肾癌细胞增殖的关系。方法 采用鼠抗人p2 7单克隆抗体及Ki- 6 7抗原单克隆抗体对 86例肾细胞癌和 86例癌旁组织进行免疫组化染色。结果 RCC中p2 7表达明显减少 ,Ki- 6 7指数 (Ki- 6 7LI)明显增高 ;且p2 7表达与Ki- 6 7指数 (Ki- 6 7LI)在肿瘤病理分级、临床分期和淋巴结转移方面呈负相关。结论 p2 7和Ki - 6 7LI从细胞生长抑制和增殖活性两个不同的侧面反映了肿瘤细胞的侵润能力 ,联合检测p2 7和Ki- 6 7LI对预测肾癌的的生物学行为及预后具有重要的意义。

Objective:To explore the expression of p27 and Ki-67 in renal cell carcinoma(RCC) and evaluate the relationship between p27 expression and tumor cell proliferation. Methods: 86 cases of RCC and 20 pericancerous tissue specimens were studied by immunohistochemistry.Results:In RCC,p27 expression decreased,while Ki-67 LI increased significantly;p27 expression was negatively related to the Ki-67 labeling index (Ki-67 LI), the tumor pathological grade, clincal stage and lymphoid node metastases.Conclusions: p27 expression and Ki-67 LI reflect RCC's growing and invasive ability from the point of proliferation and angiogenesis respectively. Combined examination of p27 and Ki-67 LI has an important siginficance in evaluating RCC's biological behavior and prognosis.