琥珀酸对中性粒细胞的抑制作用(英文)

Succinic acid inhibits polymorphonuclear leukocyte's function

目的 探讨脆弱类杆菌代谢产物琥珀酸 (succinicacid)对中性粒细胞 (polymorphonuclear,PMN)功能的影响及其在脆弱类杆菌 (bacteroidesfragilis)发病中的作用。方法 体外分离外周血PMN ,以 5 ,10 ,2 0 ,30mmol/L四种浓度的琥珀酸分别孵育 2 0min ,或以 2 0mmol/L浓度的琥珀酸孵育 5 ,10 ,15和 2 0min ,测定对PMN粘附率 (adherencerate)及硝基蓝四氮唑 (NBT)还原能力的影响。结果 琥珀酸能显著抑制PMN粘附率及NBT还原能力 ,且呈浓度 ,时间依赖关系。结论 琥珀酸能抑制PMN的功能 ,在脆弱类杆菌的发病中占有重要的作用。

Objective:To examine the effect of succinic acid on the function of human polymorphonuclear(PMN) leukocyte and it's special function as a virulent factor of Bacteroides Fragilis.Methods: PMN wasseperated from the blood of healthy donors,incubated with succinic acid at the concentration of 5,10,20 and 30 mmol/L for 20 min or at the concentration of 20 mmol/L for 5,10,15 and 20 min.Tested PMN's power of adherence and deoxidizing NBT.Results:Succinic acid can inhibit PMN's power of adherence and deoxidization,the inhibitory action was related to the time of incubation and succinic acid's concentration.Conclusions:Succinic acid can inhibit PMN's function and it may be an important virulent factor to Bacteroides Fragilis.