组织因子途径抑制物2在胰腺癌中表达的实验研究

Experimental study on expression of tissue factor pathway inhibitor-2 in human pancreatic carcinoma

目的 组织因子途径抑制物 2 (TFPI- 2 )是一丝氨酸蛋白酶抑制物 ,在维持细胞外基质完整性和调控肿瘤细胞浸润转移方面起着重要作用。分析TFPI- 2和在人类胰腺癌中表达情况 ,探讨TFPI- 2与胰腺癌恶性程度之间的相互关系 ,为临床判断胰腺癌预后及后期的基因治疗奠定基础。方法 采用WesternBlot及RT -PCR方法 ,检测TFPI- 2在 2 0例临床胰腺癌标本及 8例正常胰腺组织中的表达情况 ,HPIAS图像分析仪分析各组标本相对密度 ,t检验各组相对密度差异 ,秩和检验TFPI- 2与胰腺癌恶性程度的相关性。结果 随着胰腺癌恶性程度的加深 ,TFPI- 2表达逐渐下降 ,两者之间存在着显著的负相关性。结论 TFPI- 2与胰腺癌恶性程度之间存在着负相关性 ,可能成为胰腺癌预后的判断指标之一 ,并为基因治疗胰腺癌提供新的方向。

Objective:Human tissue factor pathway inhibitor-2(TFPI-2) is a serine protease inhibitor,which is important to maintain the integrity of extracellular matrix and regulate the migration and invasion of tumor.The correlation between TFPI-2 and the malignancy of pancreatic carcinoma was established after detected the expression of TFPI-2 level in pancreatic carcinoma and based for clinical prognosis and subsequent gene therapy of pancreatic carcinoma.Methods:Expression level of TFPI-2 in 8 normal and 20 cancerous/juxta-cancerous pancreas were textified with Western Blot and RT-PCR.Expression density of TFPI-2 in each group was analyzed with HPIAS image analyzer and compared with t test,the correlation between the expression level of TFPI-2 and malignancy was tested with Kruskal-Wallis test.Results:The expression level of TFPI-2 decreased along with the increase of malignancy,and there was a significantly negative correlation between TFPI-2 and malignancy.Conclusion:There is negative correlation between TFPI-2 and the malignancy of pancreatic carcinoma.It is suggested that TFPI-2 should be a prognostic factor and a novel approach for gene therapy.