槲皮素对烫伤大鼠血清刺激的IEC-6细胞环氧合酶活性及代谢产物的影响

Effects of quercetin on metabolism of cyclooxygenase in IEC-6 cells stimulated by the serum of burned rats

目的 观察槲皮素 (Quercetin ,Que)对烫伤大鼠血清刺激的IEC 6细胞释放PGE2 及环氧合酶 (Cyclooxygenase ,COXs)活性的影响。方法 放免法测定IEC 6细胞培养液PGE2 的含量 ,底物荧光法检测COXs酶活性的变化。结果 烫伤大鼠血清刺激可使IEC 6细胞PGE2 水平一过性增加 ,而后显著减低 ;0 .1、1.0 μmol L的Que可提高刺激后 2 4hPGE2 水平。烫伤血清刺激后IEC 6细胞COXs酶总活性变化不大 ,而COX 1 COX 2活性比显著降低 ,槲皮素对COXs活性的调节呈双向性 ,提高COX 1 COX 2活性比率。结论 槲皮素可能通过调节IEC 6细胞COXs酶的活性 ,影响其代谢产物PGE2

Objective To observe the effects of quercetin on PGE 2 release and cyclooxygenase activity in IEC 6 cells stimulated by the serum of burned rats. Methods The PGE 2 levels were measured by radioimmunoassay and the changes of cyclooxygenase activity were determined by substrate fluorescence analysis. Results Stimulated by the serum of burned rats, the PGE 2 level increased first and then obviously decreased. Quercetin at 0.1 and 1.0 μmol/L enhanced the PGE 2 level after the stimulation of IEC 6 cells for 24 h. There was no obvious change in total cyclooxygenase activity, but the activity ratio of COX 1 to COX 2 significantly decreased. Conclusion Quercetin might influence the release of cyclooxygenase metabolite PGE 2 through the regulation of COXs activity in IEC 6 cells.