非小细胞肺癌内TRAG-3抗原的表达及其临床意义

Expression of TRAG-3 antigen in non-small cell lung carcinoma and its clinical significance

目的 检测非小细胞肺癌内肿瘤抗原TRAG 3的表达情况 ,分析TRAG 3表达与术前新辅助化疗及肿瘤分期的关系。方法 用逆转录聚合酶链式反应 (RT PCR)和免疫组化的方法检测了共 48例非小细胞肺癌患者的手术切除标本中TRAG 3抗原的表达。结果 正常肺组织和癌旁组织中未见TRAG 3表达 ;48例肺癌标本中 ,共有 2 6例 ( 5 4% )非小细胞肺癌表达TRAG 3抗原 ,其中 2 2例肺腺癌中有 16例 ( 73 % )表达 ,而 2 0例肺鳞癌中有 8例 ( 17% )表达 ,两者差异显著 ;术前化疗对TRAG 3的表达无显著影响 ;TRAG 3抗原的表达率与肿瘤的分期无关。结论 非小细胞肺癌内有较高比例的TRAG 3抗原表达 ;TRAG 3抗原在非小细胞肺癌内的表达与术前化疗及肿瘤分期无关。

Objective To detect TRAG 3 expression in non small cell lung carcinoma and analyze the relationship between its expression and neoadjuvant chemotherapy and tumor grading. Methods TRAG 3 expressions in 48 surgical samples of non small cell lung carcinoma were detected by reverse transcription polymerase reaction (RT PCR) and immunohistochemistry. Results RT PCR and immunohistochemistry revealed that there was no TRAG 3 antigen expression in normal lung tissues and adjacent tissues, but 26 (54%) samples expressing TRAG 3 antigen in 48 samples of non small cell lung carcinoma, only 8 (17%) expressing TRAG 3 antigen out of 20 samples of squamous carcinoma and 16 (73%) expressing TRAG 3 antigen in 22 samples of adenocarcinoma. Preoperative chemotherapy had no effect on TRAG 3 expression. There was no statistical significance between the expression of TRAG 3 and tumor grading. Conclusion There is a high proportion of TRAG 3 expression in non small cell lung carcinomas, especially in lung adenocarcinomas. Expression of TRAG 3 in non small cell lung carcinomas is not associated with preoperative chemotherapy and tumor grading.