大黄与粉防己碱抗实验性肝纤维化的对照研究

A comparative study on the anti-hepatofibrosis mechanism of rhubarb and tetrandrine

目的 研究大黄和粉防己碱 (Tet)对大鼠实验性肝纤维化的影响并探讨其可能的作用机制。方法 四氯化碳皮下注射法制备大鼠肝纤维化模型 ,分别给予大黄和Tet灌胃。酶动力法测定肝功能 ,放免法测定细胞外基质 (ECM ) ,HE、VG染色检测肝组织病理形态学改变 ,随机抽取样本电镜下观察超微结构改变。结果 大黄与Tet均可改善肝纤维化时的肝功能状态 ,降低ECM含量 ,降低肝纤维化程度 ,以大剂量大黄和小剂量Tet效果最显著。结论 大黄与Tet可保护肝细胞 ,抑制ECM合成 ,具有防治实验性肝纤维化和肝硬化的作用。

Objective To study the anti-hepatofibrotic effects of rhubarb and tetrandrine in experimental rat model, and to explore its possible mechanism. Methods The experimental hepatic fibrosis was induced by subcutaneous injection of CCl 4. The rhubarb and tetrandrine were administered through gastric tube respectively. The liver function test was performed by enzyme kinetics and the extracellular matrix (ECM) contents were determined by radioimmunoassay. The pathological changes of liver tissues were detected by HE and VG staining; meanwhile, ultra microstructural changes were observed by electron microscope in randomly selected samples. Results Both rhubarb and tetrandrine could improve the liver function in liver fibrosis, decrease the contents of ECM and reduce the degree of liver fibrosis, with the best results in rats receiving high dosage of rhubarb and low dosage of tetrandrine. Conclusions Rhubarb and tetrandrine could protect hepatocytes, inhibit the ECM synthesis, and thereby could prevent the development of liver fibrosis and cirrhosis in the experimental animal models.