手术联合热休克蛋白70对荷瘤小鼠的治疗作用

Therapeutic Efficacy of Combination Surgical Resection and HSP70 on Tumor-bearing Mice

目的 :研究手术联合应用肿瘤来源热休克蛋白 70 (HSP70 )对荷肝癌HCaF的 6 15系小鼠的治疗作用。方法 :用液相色谱法纯化小鼠肿瘤细胞株中的HSP70 ,用SDS PAGE及Western blot对纯化产物进行定性分析 ,毛细管电泳鉴定其纯度。通过动物实验观察HSP70及手术对荷瘤小鼠的治疗作用。结果 :HSP70 10 μg组平均生存期 [>(5 9 2± 2 9 6 )d]与对照组 [平均生存期 (17 8± 3 8)d]比较差异有显著意义 ,P <0 0 2 5。手术与HSP70 10 μg联合应用组平均生存期 [>(82 0± 17 9)d]与对照组及单纯手术相比差异有显著意义 ,P <0 0 2 5、P <0 0 5。结论 :HSP70能提高生存率 ,手术与适当剂量的HSP70联合应用使生存率更加提高 。

Objective To investigate the therapeutic efficacy of treating malignant tumor with co utilization of surgery and tumor derived HSP70 on HCaF bearing 615 mice.Methods HSP70 was purified from the murine tumor cell line by liquid chromatography,and then identified by SDS PAGE and Western blot,and the purity of purified product was analyzed by capillary electrophoresis.The antitumor efficacies of HSP70 and surgery were observed by zoopery.Results Ten μg HSP70 displayed obvious therapeutic effect when singlely apllied,and prolonged the life period of the mice mean period [>(59 2±29 6)days].Co utilization of surgery and HSP70 showed the life period of the mice mean period [>(82 0±17 9)days] was significant difference from the control group and the single surgery group, P <0 025, P <0 05.Conclusions HSP70 can prolong the survival rate.Co utilizing of surgery and therapeutic dose HSP70 has apparent better therapeutic effect to tumor bearing mice than any single use. China J Cancer Prev Treat,2003,10(5):487-489