大鼠皮层C6脑胶质瘤模型的建立

Establishment of Rat Cortex C6 Brain Glioma Model

目的 :为脑胶质瘤的研究提供实验基础 ,建立稳定可靠的动物模型。方法 :借助于脑立体定位技术 ,在 2 5只雄性Wistar大鼠脑S1区接种含有 10g L琼脂糖的C6细胞悬液。随机分成 4个周组和 1个自然死亡组后观察大鼠的生存期 ,并采用MRI、病理解剖、HE染色及GFAP免疫组织化学方法 ,动态观察各周组肿瘤生长情况。结果 :在不同周组中 ,所有检查均显示颅内肿瘤形成 ,GFAP阳性。 4周内无死亡 ,自然死亡组在第 5周死亡 2只、余 3只于第 6周死亡。结论 :该方法建立的S1区脑胶质瘤模型具备与人脑胶质瘤生长相似的特性 ,肿瘤形成时间短 ,生存期稳定 ,且无脑外转移和颅外扩散 ,适合各种脑胶质瘤实验治疗研究。

Objective To establish a stable and dependable animal model as a laboratory foundation for treatment research in human brain glioma.Methods By stereotaxic technique,25 male Wistar rats were inoculated with C6 cell suspension containing 10 g/L agarose in their brain S1 area.After being randomized to 4 week groups and 1 natural death group the rats were observed of survival period and the examinations such as MRI,patholoanatomy,HE stain and GFAP immunohistochemistry were prformed on each week group rats to observe dynamically the tumor growth.Results Intracranial tumors existed in different week groups.Expression of GFAP was positive.The death was not detected in 4 weeks.However,in natural death group,two rats died in No.5 week and the others died in No.6 week.Conclusions The S1 area brain glioma model established by this method in rats had the same characters as human brain glioma.The tumor growth period was short and the subsisting period was stable without extracerebral metastasis and extracranial extension.This model is suitable for laboratory and treatment study of brain gliomas. China J Cancer Prev Treat,2003,10(5):490-492