Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells

AIM: To investigate the effects of tachyplesin on the cell cycle regulation in human hepatcarcinoma cells.METHODS: Effects of tachyplesin on the cell cycle in human hepatocarcinoma SMMC-7721 cells were assayed with flow cytometry. The protein levels of p53, p16, cyclin D1 and CDK4 were assayed by immunocytochemistry. The mRNA levels of p21WAF1/CIP1 and c-myc genes were examined with in situ hybridization assay.RESULTS: After tachyplesin treatment, the cell cycle arrested at G0/G1 phase, the protein levels of mutant p53, cyclin D1 and CDK4 and the mRNA level of c-myc gene were decreased, whereas the levels of p16 protein and p21wWF1/CIP1 mRNA increased.CONCLUSION: Tachyplesin might arrest the cell at G0/G1 phase by upregulating the levels of p16 protein and p21WAF1/CIP1 mRNA and downregulating the levels of mutant p53, cyclin D1 and CDK4 proteins and c-myc mRNA, and induce the differentiation of human hepatocacinoma cells.

AIM;TO investigate the effects of tachyplesin on the ce cycle regulation in human hepatcarcinoma cells. METHODS:Effects of tachyplesin on the cell cycle in human hepatocarcinoma SMMC-7721 cells were assayed with flow cytometry.The protein levels of p53,p16,cyclin D1 and CDK4 were assayed by immunocytochemistry.The mRNA levels of p21^(WAF1/CIP1)and c-myc genes were examined with in situ hybridization assay. RESULTS:After tachyplesin treatment,the cell cycle arrested at G_0/G_1 phase,the protein levels of mutant p53, cyclin D1 and CDK4 and the mRNA level of c-myc gene were decreased,whereas the levels of p16 protein and p21^(WAF1/CIP1)mRNA increased. CONCLUSION:Tachyplesin might arrest the cell at G_0/G_1 phase by upregulating the levels of p16 protein and p21^(WAF1/CIP1)mRNA and downregulating the levels of mutant p53,cyclin D1 and CDK4 proteins and c-myc mRNA,and induce the differentiation of human hepatocacinoma cells.