摘要
AIM: To investigate the correlation between gammaglutamyl transpeptidase (γ-GTP) expression in the primary HCC and post-resection recurrence and its biological behaviors.METHODS: Forty consecutive patients having curative resection for HCC were included in this study. The primers for reverse-transcription polymerase chain reaction (RTPCR) were corresponding to the 5'-noncoding human γ-GTP mRNA of fetal liver (type A), HepG2 cells (type B),and placenta (type C). Both the cancer and non-cancerous tissues of the resected liver were analyzed. The correlations between the expression of γ-GTP and the clinicopathological variables and outcomes (recurrence and survival) were studied.RESULTS: Those with type B γ-GTP mRNA in cancer had significant higher recurrence rate than those without it (63.6 % vs 14.3 %). Both those with type B in cancer and in non-cancer died significantly more than those without it (45.5 % vs 0 % and 53.6 % vs 0 %, respectively). By multivariate analysis, the significant predictors of recurrence included high serum AFP (P=0.0108), vascular permeation (P=0.0084), and type B γ-GTP mRNA in non-cancerous liver (P=0.0107). The significant predictors of postrecurrence death included high serum AFP (P=0.0141),vascular permeation (P=0.0130), and daughter nodules (P=0.0053). As to the manifestations (recurrent number ≥2, recurrent extent≥2 segments, extra-hepatic metastasis, and death) in recurrent patients, there were no statistical significant differences between those with type B in the primary tumor and those without it. The difference between those with type B in non-cancerous liver and those without it also was not significant.CONCLUSION: Patients of HCC with type B γ-GTP mRNA both in cancer and in non-cancerous tissue had a worse outcome, earlier recurrence, and more post-recurrence death.
AIM;To investigate the correlation between gamma- glutamyl transpeptidase(γ-GTP)expression in the primary HCC and post-resection recurrence and its biological behaviors. METHODS:Forty consecutive patients having curative resection for HCC were included in this study.The primers for reverse-transcription polymerase chain reaction(RT- PCR)were corresponding to the 5'-noncoding human y- GTP mRNA of fetal liver(type A),HepG2 cells(type B), and placenta(type C).Both the cancer and non-cancerous tissues of the resected liver were analyzed.The correlations between the expression of γ-GTP and the clinicopathological variables and outcomes(recurrence and survival)were studied. RESULTS:Those with type B γ-GTP mRNA in cancer had significant higher recurrence rate than those without it (63.6 % vs 14.3 %).Both those with type B in cancer and in non-cancer died significantly more than those without it (45.5 % vs 0 % and 53.6 % vs 0 %,respectively).By multivariate analysis,the significant predictors of recurrence included high serum AFP(P=0.0108),vascular permeation (P=0.0084),and type B γ-GTP mRNA in non-cancerous liver(P=0.0107).The significant predictors of post- recurrence death included high serum AFP(P=0.01.41), vascular permeation(P=0.0130),and daughter nodules (P=0.0053).As to the manifestations(recurrent number ≥2,recurrent extent>2 segments,extra-hepatic metastasis,and death)in recurrent patients,there were no statistical significant differences between those with type B in the primary tumor and those without it.The difference between those with type B in non-cancerous liver and those without it also was not significant. CONCLUSION:Patients of HCC with type B γ-GTP mRNA both in cancer and in non-cancerous tissue had a worse outcome,earlier recurrence,and more post-recurrence death
基金
grants from the Department of Health,National Science Council,Executive Yuan,Taiwan.NSC 87-2314-B-195-003
