同型半胱氨酸对大鼠血管平滑肌细胞胶原蛋白合成及其mRNA表达的影响

Effect of Homocysteine on Collagen Synthesis and Expression ofType Ⅰ Collagen mRNA of Vascular Smooth Muscle Cells in Rats

目的 :观察同型半胱氨酸 ( Hcy)对血管平滑肌细胞 ( VSMCs)胶原合成的影响 ,并探讨其作用机制。方法 :本实验采用体外培养的大鼠 VSMCs,加入不同浓度的 Hcy,通过 3 H-脯氨酸掺入、α1[ ]型胶原测定观察 Hcy对 VSMCs胶原合成的影响 ,并通过半定量 RT- PCR检测其对 α1[ ]型胶原 m RNA表达的影响。结果 :Hcy促进 VSMCs的胶原合成及 α1[ ]型胶原 m RNA表达 ,并呈剂量依赖效应。结论 :Hcy促进 VSMCs的胶原合成 ,可能是通过促进 α1[ ]型胶原 m RNA表达实现的。提示

Objective: To study the effect of homocysteine(Hcy)on collagen synthesis and expression of type Ⅰ collagen mRNA of vascular smooth muscle cells(VSMCs) in culture. Methods: To culture VSMCs of rat with different amount of Hcy in vitro. 3H proline incorporation and type Ⅰ collagen measurement and semi quantitative RT PCR were performed. Results: Hcy enhanced collagen synthesis and upregulated expression of type Ⅰcollagen mRNA of VSMCs in a dose dependent manner. Conclusion: Hcy may promote collagen synthesis through upregulating type Ⅰ collagen mRNA expression in VSMCs in culture. These data indicated that enhancement collagen synthesis may be one of the mechanisms of the pathogenesis of atherosclerosis induced by Hcy.