摘要
目的 成功构建带有视黄酸反应元件 (RARE) TK启动子控制的增强型绿色荧光蛋白 (eGFP)报告基因表达载体 ,并验证该载体转染HL6 0细胞后全反式视黄酸 (ATRA)依赖的eGFP表达。方法 采用常规分子克隆方法构建真核表达载体pRARE TK eGFP ,经脂质体转染肿瘤细胞 ;ATRA处理后检测细胞增殖功能变化和经荧光显微镜直接观测eGFP表达变化情况。结果 ATRA处理转染HL6 0细胞后 ,eGFP呈视黄酸 (RA)时间剂量依赖性表达增高。结论 ATRA可通过RARE调控TK启动子下游的eGFP报告基因表达。
ObjectiveTo investigate the retinoic acid-dependent expression of enhanced green fluorscent protein (eGFP) after transduced plasmid (RARE-TK-eGFP) in HL-60 cells. MethodsMammalian vector pRARE-TK-eGFP was constructed and transduced to HL60 cells with liposome DOTAP. The change of expression of eGFP was observed by fluorescence microscope analysis. ResultsThe expression of eGFP was dramatically related to the effect of RA in HL60 cells. ConclusionThere was RA-dependent expression of eGFP gene by RARE-TK promoter.
出处
《肿瘤》
CAS
CSCD
北大核心
2003年第3期180-182,共3页
Tumor
基金
国家自然科学基金资助项目 (编号 :3 9970 3 2 1)