乳腺癌端粒酶活性及多项生物学指标表达与临床相关性分析研究

Analytical study on clinical correlation between telomerase activity and biological indices in breast cancer

目的 研究乳腺癌组织中端粒酶活性的表达,探讨端粒酶活性与ER、PR、CerbB-2、PCNA及Cath-D的相关性,分析其与乳腺癌肿瘤大小、临床分期、病理组织学分型及淋巴结转移的关系。方法 采用PCR-ELISA法和免疫组化技术,检测乳腺癌组织中的端粒酶活性,ER、PR、CerbB-2、PCNA及Cath-D的蛋白表达水平。结果 在乳腺癌组织中端粒酶活性、ER、PR、CerbB-2、PCNA及Cath-D阳性表达率分别为82.0％、67.5％、65.0％、65.0％、77.5％、52.5％。端粒酶活性与乳腺癌肿瘤大小、临床分期、淋巴结转移有相关性,与病理组织学分型无相关性。端粒酶活性与ER有相关性,与PR、CerbB-2、PCNA、Cath-D的表达无相关性。结论 端粒酶活性与ER水平、肿瘤大小、临床分期、淋巴结转移有相关性,可作为评估乳腺癌预后的分子生物学指标。

Objectives To study the expression of telomerase activity in breast cancer and its clinical correlation with ER,PR,CerbB-2,PCNA and Cath-D,and to analyze its correlation with the tumor size,clinical stage, histopathological typing and lymphatic metastasis. Methods PCR-ELJSA and immmunohistochemical staining were used to detect the telomerase activity and expression of ER,PR, CerbB-2, PCNA and Cath-D in the tissues of breast cancer. Results The expression rate of telomerase activity was 82.0 % , ER was 67.5 % , PR was 65.0 % , C-erbB-2 was 65.0%, PCNA was 77.5% and Cath-D was 52.5% .Telomerase activity was correlated with tumor size, clinical stage, lymphatic metastasis, and not correlated with histopathological typing. Telomerase activity was correlated with ER.and not correlated with PR, CerbB-2, PCNA and Cath-D. Conclusion The results suggest that telomerase activity may be a marker of breast cancer.