超临界流体色谱测定牛奶中乳清酸的含量

Determination of Orotic Acid in Cow Milk by Supercritical Fluid Chromatography

使用超临界流体色谱测定牛奶中乳清酸含量,在二氧化碳流动相中添加20%的甲醇及0.25%三氟乙酸作改性剂,于填充柱上分离,检测波长为280nm,在测定范围内,对照品浓度与峰面积呈良好的线性关系(r=0.9991)。以峰面积和保留时间计算精密度分别为2.33%和0.47%。回收率为101.9%±1.92%,3min即可完成色谱分析,样品前处理简单,可用于牛奶中乳清酸的快速测定。

A rapid SFC method for determination of orotic acid in cow milk is described. The mobile phase was a mixture of CO2and methanol (contained 0.25% TFAA) at a rate of 2.0ml/min,and a packed column was used. The UV detector was at awavelength of 280nm.The calibration curve was linear in the range of determination(r=0.9991). The RSD of peak area andretention time were 2.33% and 0.47% respectively. The recovery was 101.9%±0.92%. The analysis was completed within 3minutes. The method was simple and it was easy for handling sample. It could be used in rapid analysis.