银杏黄酮对慢性酒精所致大鼠氧化损伤保护作用的研究

The Protective Effects of Flavonoids of Ginkgo Biloba on Rat Oxidative Damage Caused by Chronic Alcohol Intakes

目的 研究银杏黄酮对慢性酒精中毒大鼠氧化损伤的保护作用。 方法 大鼠酒精灌胃,银杏黄酮采用预防性给药的方法。于实验第30d、60d分别检测大鼠血清中谷丙转氨酶(Glutamic-Pyruvic Transaminase, ALT)、谷草转氨酶(Glutamic Oxaloacetic Transaminase, AST)活性;谷胱甘肽(Glutathione, GSH)、活性氧(Reactive Oxygen Species,ROS)以及丙二醛(Malondialdehyde, MDA)的含量。 结果 慢性酒精摄入30 d和60 d后,酒精组血清ROS、ALT、AST和MDA较对照组明显升高,差异有极显著性(p<0.01),GSH则显著性降低 (p<0.01);与此同时,实验第30 d和60 d,低、高剂量银杏黄酮能明显降低酒精所致的ROS、ALT、AST、MDA升高(p<0.01),第60 d,高剂量银杏黄酮能显著抑制酒精所致的GSH降低(p<0.01)。结论 银杏黄酮对酒精所致氧化损伤具有一定的保护作用。

Objective To study the protective effects of flavonoids of ginkgo biloba on chronic alcohol induced oxidative damageon rat. Methods The rat was treated by gastric incubation with alcohol. After administered with preventive medicine flavonoidsof ginkgo biloba, the activity of ALT(Glutamic-Pyruvic Transaminase, ALT)、AST(Glutamic Oxaloacetic Transaminase, AST)were measured in rat serum, meanwhile, the concentration of ROS(Reactive Oxygen Species)、 Glutathione (GSH)、Malondialdehyde (MDA) on the 30th day and 60th day of the experiment, respectively were determined to indicate serumoxidative-damage. Result The preventive experiment display that on the 30th day, the low and high doses flavonoid of ginkgobiloba can significantly decrease the high ROS、ALT、AST and MDA levels induced by alcohol (p<0.01);moreover, the highdose can increase GSH level reduced by alcohol markedly(p<0.01) on the 60th day. Conclusion flavonoids of ginkgo biloba havesome protective effects on chronic alcohol induced oxidative damage on rat serum.