柚皮提取物抗氧化作用和对正常小鼠免疫功能的影响

Studies on the Antioxidant Activity of Shaddock Peels Extract and Its Effect on the Immunomodulatory System of Normal Mice

研究了柚皮提取物的抗氧化作用和对正常小鼠免疫功能的影响,结果表明柚皮提取物在一定浓度下可以有效的抑制菜油的氧化,但是不具有剂量-效应关系。500×10-6的柚皮提取物抗氧化效果最好,并强于1000×10-6的VC和BHT,但柚皮提取物在高浓度时 (1000×10-6、2000×10-6)反而有轻微的助氧化作用。抗坏血酸对柚皮提取物抗氧化效果具有明显的协同增效作用;柚皮提取物可明显增强正常小鼠细胞免疫和非特异性免疫功能,能显著地提高正常小鼠血清SOD的活性、降低血清MDA的含量,且呈明显的剂量-效应关系。

The antioxidant activity of shaddock peels extract(SPE) in rape oil system and its immunomodulatory effects on normalmice was studied. Grapefruit Skin extract showed significant antioxidant activity in rape oil system at concentration of 500×10-6,and VC had synergistic antioxidant effect to SPE in rape oil system. but when the concentration increased to 1000×10-6 and 2000×10-6, it showed pro-oxidant effect, and SPE can also activate serum SOD and reduce the content of MDA of mice significantly,and the concentration-effect relationship was obvious; It was found that Grapefruit Skin extract (Po,100,200mg/kg·d×7d)can significantly intensify DTH caused by DNFB, and SPE (100mg/kg·d,200mg/kg·d, ×15d) can improve phagocytosingfunction of peritoneal macrophage(PMφ) by 22.94%, 53.96% respectively;