病毒性心肌炎患儿血清干扰素-γ、肿瘤坏死因子-α的变化及与心肌酶异常的关系

The changes of interferon-γ and tumor necrosis factor-α in childr en with viral myocarditis and the relationship with abnormal cardiac enzyme

目的 探讨干扰素 γ (IFN γ)、肿瘤坏死因子 α(TNF α)在病毒性心肌炎 (VM)发病中的作用。方法 采用双抗体夹心ELISA法测定 50例VM患儿及 50例健康正常儿童外周血清IFN γ、TNF α含量 ,采用美国En coreTH全自动生化分析仪检测心肌酶 ,并分析IFN γ、TNF α与心肌酶异常的关系。结果 VM组IFN γ、TNF α含量均显著高于健康正常儿童 (P均 <0 .0 1) ;VM组IFN γ、TNF α升高者 ,各项心肌酶异常百分数均增高 (P <0 .0 5)。结论 IFN γ、TNF α参与VM的发病 ;检测VM患儿IFN γ、TNF α含量有助于VM的诊断、判断病情和预后 。

Objective To investigate the roles of cytokine tha t interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) play in CoxB vir al myocarditis.Methods The peripheral blood of 50 cases with Co xB viral myocarditis and 50 normal children were collected. The levels of IFN- γ?TNF-α were measured by ELISA. The cardiac enzymes of 50 cases with CoxB vi ral myocarditis were measured. And the relationship between the changes of cytok ine IFN-γ,TNF-α and the cardiac enzymes were analyzed.Results The levels of IFN-γ and TNF-α were significantly hi gher in viral myocarditis than those in normal children (P<0.01.) The perc entage of cardiac enzyme abnormity from the patients whose IFN-γ and TNF-α w ere elevated increased (P<0.05.) Conclusions IFN-γ and TNF-α may play a role in viral myocarditis. IFN-γ and TNF-α are helpful fo r diagnosing,observing curative effects and prognosis of viral myocarditis and i t provided a new clue to the treatment of VM.