抑癌基因PTEN在食管癌中的表达及其临床意义

Expression and clinical significance of PTEN in esophageal cell carcinoma

目的 研究食管癌中第 10号染色体丢失的张力蛋白同源的磷酸脂酶基因 (PTEN基因 )的表达及其临床意义。方法 用免疫组化方法检测 68例食管癌和癌旁正常组织中PTEN蛋白的表达水平。结果 食管癌中PTEN蛋白表达率为 70 .6% ,而癌旁正常组织为 10 0 % ,PTEN蛋白表达与肿瘤分化程度、浸润深度、淋巴结转移及TNM分期相关 (P <0 .0 5 )。PTEN蛋白在高中低分化组的阳性表达率分别为 90 .17%、66.67%和 5 0 %。单变量分析PTEN蛋白阳性表达组 3、5年生存率分别为 70 .3 %和 5 4.6% ,高于阴性表达组的 40 .2 5 %和 16.1%。经long rank时序检验差异有显著性(P =0 .0 2 5 8) ,Cox回归模型多变量分析显示 ,PTEN是一个独立预后指标 (P =0 .0 12 )。结论 从蛋白水平证明PTEN基因表达缺失或降低与食管癌的发生发展有关 。

Objective To investigate the significance of expression of phosphatase and tensin homologue derived from chromosome ten (PTEN) in esophageal squamous cell carcinoma.Methods Immunohistochemical staining was used to determine the expression of PTEN in 68 cases of esophageal squamous cell carcinoma and normal esophageal tissues closely adjacent to carcinoma.Results The incidence of PTEN in esophageal cell carcinoma was 70.6% while in normal esophageal tissue it was 100%.The expression of PTEN was correlated with degree of differentiation of carcinoma,lymph node metastasis and TNM Stage.The expression rates of PTEN in highly,moderately and poorly differentiated groups were 90.17%,66.67% and 50%,respectively. Univariate analysis showed that the 3 and 5 year overall survival rates in PTEN expression group were 70.3%、54.6% and in the negative group were 40.3%,16.1% respectively.There was significant difference between two groups(P=0.0258).On multivariate analysis by Cox regression model PTEN expression was of independent prognostic significance.Conclusion PTEN gene plays an important role in carcinogenesis and progress of esophageal cell squamous carcinoma.Expression of PTEN may prove to be valuable in prognosis assessment of esophageal squamous cell carcinoma.