叶酸对胃癌前病变bcl-2、bax及p53基因的影响

Effects of folic acid on the expressions of bcl-2, bax and p53 gene in precancerous condition of gastric carcinoma

目的 研究叶酸治疗对胃癌前病变组织中bcl 2、bax及p5 3基因表达的影响。方法 胃镜活检经病理证实为胃癌前病变患者 3 8例 ,利用逆转录聚合酶链式反应 (RT PCR)方法检测胃癌前病变组织bcl 2、bax基因表达率 ,利用流式细胞仪检测组织p5 3蛋白表达率。将患者随机分为治疗组 (叶酸 10mg ,每天三次 )与对照组 (硫糖铝 1.0 ,每天四次 )各 19例 ,治疗结束复查组织中bcl 2 ,bax基因表达率及p5 3蛋白表达率。结果 治疗组治疗后bcl 2基因表达率降低 (P <0 .0 5 ) ,bax基因表达率无明显变化 (P >0 .0 5 ) ,p5 3蛋白表达率增高 (P <0 .0 5 ) ,对照组治疗后各项指标无明显变化 (P >0 .0 5 )。结论 叶酸干预可促进胃癌前病变组织中p5 3基因的表达 ,抑制bcl 2基因的表达 。

Objective To study the effects of folic acid on the expressions of bcl-2,bax and p53 genes in precancerous condition of gastric carcinoma.Methods Gastric biopsy specimens were taken by endoscopy from 38 patients with precancerous condition.The expressions of bcl-2 gene and bax gene were determined by RT-PCR, the expression of p53 gene was determined by flow cytometry.The patients were randomly divided into treatment group(n=19,folic acid 10mg,tid)and control group(n=19,sucralfate 1.0,qid).After therapy the expressions of bcl-2,bax and p53 were reexamined.Results After therapy of folic acid the expression of p53 gene was increased(P<0.05),the expression of bcl-2 gene was decreased(P<0.05),the expression of bax gene was not changed(P>0.05).Conclusions Folic acid may promote p53 gene expression and inhibit Bcl-2 gene expression,but no effect on bax gene expression.