尿转化生长因子-β1的检测在IgA肾病纤维化中的临床意义

The significance of urine transforming growth factor-β1 in renal fibrosis of lgA nephropathy

目的 探讨检测尿转化生长因子 β1(TGF β1)在lgA肾病纤维化中的临床意义。 方法 采用双抗体夹心ELISA法、天狼猩红染色后偏光镜、免疫组织化学方法检测 3 0例IgA肾病、5例正常人尿中TGF β1水平 (用同一尿标本Cr水平作标准比 )及肾小球和肾小管中Ⅰ、Ⅲ、Ⅳ型胶原水平。结果 lgA肾病各组尿TGF β1/Cr明显高于对照组 (P <0 .0 1)。其中中、重度组明显高于轻度组及对照组 (P值均 <0 .0 1)。尿TGF β1/Cr水平与肾小球Ⅰ、Ⅲ、IV型胶原 ,肾小管中Ⅰ、Ⅲ、IV型胶原、2 4小时尿蛋白呈显著正相关 (r值分别为 0 .85 5 ,0 .82 6,0 .92 ;0 .92 2 ,0 .862 ,0 .886;0 .86。P均<0 .0 1)。结论 尿TGF

objective To study the relationship between the concentration of urine transforming growth factor-β1 and renal fibrosis in lgA nephropathy.Methods Sandwich enzyme linked immunosorbent assay(ELISA),sirius red staining polarization microscopy,immunohistochemistry method and computer imaging analysis system were used to detect the urine TGF-β1,collagen Ⅰ,collagen Ⅲ,collagen IV and their relationship each other. Result In moderately advanced and far advanced lgA nephropathy,the levels of urine TGF-β1/Cr were higher than those in healthy subjects and mild groups(P<0.01)and urine TGF-β1/Cr also showed a positive relation with collagen Ⅰ,collagen Ⅲ,collagen IV in the kidney and 24 hour urinary protein.Conclusion Higher urine TGF-β1/Cr levels correlate with renal fibrosis,so it may be used as an useful marker for renal fibrosis activity.