腭扁桃体中β-防御素的表达及意义

Expression of human β-defensin in palatine tonsil

目的 :检测人β 防御素 (hBD) 1,2mRNA在慢性扁桃体炎组织和正常对照组织中的表达 ,分析腭扁桃体在天然免疫中的作用。方法 :应用逆转录聚合酶链反应技术检测 10例扁桃体炎组和 8例正常对照组中hBD 1和hBD 2mRNA的表达。结果 :hBD 1mRNA在扁桃体炎组和正常对照组中均有表达 ,且两组间差异无统计学意义 (P >0 .0 5 )。hBD 2mRNA在正常对照组中仅有微弱表达 ,而在扁桃体炎组中表达明显增强 (P <0 .0 5 )。结论 :扁桃体通过表达hBD在天然免疫中发挥重要作用。

Objective:To examine the expression of human β-defensin-1 (hBD-1) and human β-defensin-2 (hBD-2)in palatine tonsil of normal subjects and patients with chronic tonsillitis,and to investigate the role of palatine tonsil in the innate immunity system.Method: Eighteen specimens of hyperplastic tonsils (n=8) and chronic tonsillitis(n=10) were examined using reverse transcriptase-polymerase chain reaction (RT-PCR). Result:β-defensin 1 mRNA was expressed in all tissue specimens, at levels that did not differ significantly (P> 0.05 ). β-defensin 2 mRNA was detected in the specimens of chronic tonsillitis, but almost not in the normal subjects .Its expression level was significantly higher in chronic inflamed tonsils than in normal controls(P< 0.05 ). Conclusion: These results suggest that beta-defensin of the palatine tonsil may play an important role in innate defense against microorganisms.