整合素α6 β4在人喉癌细胞中的表达及对肿瘤细胞黏附与浸润性状的影响

Expression of the integrin α6 β4 and effects of integrin α6 β4 on adhensive and invasive behaviors of human laryngeal carcinoma cell lines

目的 :探讨整合素α6、β4在人喉癌细胞Hep 2和AMC HN 8中的表达及其对肿瘤细胞体外黏附性状的影响及机制。方法 :应用流式细胞术检测Hep 2和AMC HN 8人喉癌细胞株整合素α6、β4的表达 ;采用整合素亚基α6、β4特异性单抗对喉癌细胞进行黏附抑制试验与快速体外浸润实验。结果 :Hep 2和AMC HN 8细胞株均表达较高的整合素α6、β4 ,其阳性细胞百分率分别为 (4 5 .73± 2 .31) %、(2 5 .76± 6 .6 3) %和 (4 5 .5 2± 15 .5 2 ) %、(84 .5 0± 16 .5 4) %。α6特异性单抗对Hep 2细胞株有明显黏附抑制作用 (P <0 .0 5 ) ,β4特异性单抗则无明显黏附抑制作用 (P>0 .0 5 ) ,抗α6加抗β4黏附抑制作用更为明显 (P <0 .0 1) ;抗α6或抗 β4对AMC HN 8细胞株均有明显黏附抑制作用 (均P <0 .0 5 ) ,抗α6加抗 β4其抑制作用更明显 (P <0 .0 1)。结论 :整合素α6、β4在喉癌细胞的黏附和浸润过程中起重要作用。利用α6、β4特异性单抗封闭喉癌细胞表面的α6、β4后可抑制肿瘤细胞的黏附与浸润。

Objective:To study the expression of integrin α6 β4 in human laryngeal carcinoma cell lines Hep-2 and AMC-HN8. To explore the mechanism and the effects of integrin α6 β4 in human laryngeal carcinoma cell lines.Method:Flowcytometry was used to exame the surface expression of integrin α6 β4 in human laryngeal carcinoma cell lines.Anti-α6 and anti-β4 specific monoclonal antibody were used in adherence inhibition assay and in vitro invasion assay.Result:Overexpression of integrin α6 β4 were present in both Hep-2 and AMC-HN8 cell lines. Anti-α6 and anti-β4 specific monoclonal antibody together blocked the α6 β4 on the surface of the cells resulting in low adherence on the extracellular matrix and weak invasion through the extracellular matrix.Conclusion:Integrin α6 β4 play a vitral role in the adhension and invasion of laryngeal carcinoma .Anti-α6 and anti-β4 specific monoclonal antibody together can block the function of integrin α6 β4.So treatment with anti-α6 and anti-β4 specific monoclonal antibody may reduce the development of laryngeal carcinoma.