二苯三硝基苯肼对耳蜗微循环的影响

The effects of DPPH on cochlear microcirculation

目的 :观察二苯三硝基苯肼 (DPPH)对耳蜗微循环的影响。方法 :2 0只豚鼠随机分为对照组 (6只 ) ,1mmol/L组 (6只 ) ,0 .1mmol/L组 (8只 )。将药液 2 μl滴于圆窗膜。采用激光多普勒流量仪监测耳蜗血流量、运动细胞数目、血流速度 ;运用压力传感器及前置放大器记录平均动脉血压 ,经A/D转换器同步输入计算机 ,供统计、分析。结果 :对照组耳蜗微循环及平均动脉血压无显著性变化 ,1mmol/L组可见耳蜗血流量增加 12 .4 9% (P <0 .0 5 ) ,0 .1mmol/L组耳蜗血流量下降 3.0 2 % (P <0 .0 5 ) ,余无显著性变化。结论 :DPPH对耳蜗微循环有影响 ,浓度高时增加血流 ,低时降低血流。

Objective:To investigate the effects of free radical, 1, 1-Diphenyl-2-picylhydrazyl(DPPH), on cochlear blood flow.Method:Twenty guinea pigs were divided into 3 groups at random, 6 for control group, 6 for 1 mmol/L of DPPH group and 8 for 0.1 mmol/L of DPPH group. 2 μl vehicle or drugs were dropped into round window membrane. Cochlear microcirculation was monitored by laser Doppler flowmeter and the mean arterial blood flow(MABP), which transferred by pressure conductor sensor and preamplifier, was simutaneously recorded by a computer.Result:MABP was stable throught the experiment. Cochlear blood flow increased 12.49 %(P< 0.05 ) in 1 mmol/L group, and decreased 3.02 %(P< 0.05 ). In control group cochlear microcirculation had no significant changes.Conclusion:DPPH has effects on cochlear microcirculation. The mechanism still need to be explored later.