安徽药菊叶片直接再生植株技术的研究

Studies on the technology of directly inducing regenerated plantlet from leaf of Chrysanthemum morifolium

目的 :建立诱导安徽药菊叶片直接再生植株的最佳培养基。方法 :从无菌脱毒苗上采取叶片 ,以不同方式接种到附加不同植物生长物质的培养基中 ,诱导叶片直接再生植株。结果与结论 :对亳菊、滁菊来说 ,培养基MS +6 BA 0 .1mg·L-1+NAA 0 .1mg·L-1诱导效果最好 ;其中以背接方式适于植株的直接再生。

Objective: To set up the fittest medium for directly inducing regenerated plantlet from leaf of Chrysanthemum morifolium. Method: Leaves of different phases obtained from the virus-free plantlets were cut and cultured in some kinds of media. Result and Conclusion: To Bo morifolium and Chu morifolium,the fittest medium for the formation of regenerated plantlet was MS+6-BA 0.1 mg·L -1 +NAA 0.1 mg·L -1 ,and to be incubated on the right side was better than on the back right.