用高效离子交换色谱法分析使君子药材中胡芦巴碱成分

IE-HPLC analysis of trigonelline in Fructus Quisqualis

目的 :运用高效离子交换色谱法测定使君子药材中胡芦巴碱成分的含量。方法 :色谱柱为SpherisorbSCX(4.6mm× 15 0mm ,5 μm) ,流动相为 2 0mmol·L-1磷酸盐溶液 (2 0mmol·L-1磷酸二氢钠溶液用 2 0mmol·L-1磷酸溶液调节pH至 3.2 ) ,检测波长 2 6 5nm ,流速 1.0mL·min-1,柱温 2 5℃。结果 :选用SCX柱 ,使君子药材中胡芦巴碱成分可得到较好分离 ,胡芦巴碱进样量在 0 .0 5 96～ 1.788μg呈良好线性关系 ,方法平均回收率为 99.4 % (n =6 )。实验另发现使君子果皮中不含胡芦巴碱成分。结论 :本方法的建立可用于控制使君子药材的质量。

Objective: To establish a IE-HPLC method for the determination of trigonelline in the fruit and seed of Quisqualis indica . Method: The method was carried out by using Sperisorb SCX column with a mobile phase consisting of 20 mmol·L -1 phorsphate(pH3.2,titrate a 20 mmol·L -1 monsodium salt with a 20 mmol·L -1 solution of phosphoric acid),flow rate of 1.0 mL·min -1 ,and the detection at 265 nm. Result:By using SCX-modified silica column,trigonelline in Fructus Quisqualis can be baseline separated and determinated. The retention methanisms proved to be contributed mainly by ion-exchange with the SCX moieties. The validity of methods was demonstrated with respect to linearity,precision,reproducibility and recovery. It was found that there was no trigonelline occurring in the peel of Fructus Quisqualis. Conclusion: This assay method can be used for the quality control of Fructus Quisqualis.