高效毛细管电泳法测定血清中头孢他啶的含量

Determination of ceftazidime in serum by HPCE

采用高效毛细管电泳法测定血清中头孢他啶的含量。毛细管 6 0cm× 75 μm ;运行缓冲液 2 5mmol/L四硼酸钠 (pH8 2 ) ,高压进样 5s,分离电压 2 0kV ,温度为 2 5℃ ,检测波长为 2 5 4nm ,地塞米松磷酸钠为内标。头孢他啶在 0 .6 2 5～ 2 0 μg/mL浓度范围内线性关系良好 (r =0 .9997) ,平均回收率为 98.81 % (n =5 ,RSD =1 .88% )。

A HPCE method for the determination of the content of ceftazidime in serum was used. The best condition was achieved with a fused silica capillary tube (60 cm×75 μm), 25 mmol/L sodium tetraborate buffer (pH 8.2), at a constant voltage of 20 kV and temperature at 25 ℃. The UV detection wavelength was at 254 nm and dexamethasone sodium phosphate as internal standard. The calibration curves was linear in the range of 0.625～20 μg/mL for ceftazidime( r =0.999 7)and the average recovery of ceftazidime was 98.81%( n=5,RSD =1.88%).