HPLC法测定盐酸克仑特罗片的含量

Determination of clenbuterol hydrochloride Tablets by HPLC

目的:建立 HPLC 法测定盐酸克仑特罗片的含量。方法:采用 YWG-C_(18)色谱柱(150mm×6.0mm,粒度:10μm)。以甲醇-水-磷酸-三乙胺(50:50:0.2:0.4)为流动相,流速为0.4ml/min,检测波长为243nm,柱温为室温。结果:盐酸克伦特罗的线性范围为5～25μg/ml(r=0.9997),样品溶液在6h 内稳定,平均回收率为96.65%,RSD=0.56%。结论:本法简便快捷,精密度好,结果准确。

Objective To extablish HPLC method for deter-mination of clenbuterol hydrochlo- ride Tablets Methods The YWG-C18 as mobile phase and methanol-water-Phosphoric acid Thi- ethylamine(50:50:0.2:0.4,V/V/V/V).The detection wavelength was 243nm.The flow rate was 0.4ml/min.Result:The linear relationship of Calibration curve was good in the range be- tween 5～25/μg/ml,r=0.9997).The average recovery and RSD were 96.65% and 0.5%(n=5). The precision of 6h RSD was 0.56%.Conclusions:this method was simple,rapid and accurate.