摘要
研究了降温速率、保护剂种类与浓度、复溶溶液对HB-Ⅰa冻干脂质体粒径的影响。由逆相蒸发法制得的HB-Ⅰa脂质体大多为大单室和多室脂质体,粒径主要分布在1～10mm之间。约20K/min的降温速率对HB-Ⅰa脂质体冻干较为有利;蔗糖的保护效果较优,甘露醇次之,PVP和甘氨酸相对较差,蔗糖和甘氨酸组成的二元保护剂保护效果较蔗糖更好;缓冲溶液较纯水或蔗糖溶液的复溶效果好。
The influences of the cooling rate, cryoprotectants and rehydration solutions on the vesicles size anddiameter distribution of HB-Ⅰa (an antitumor effective glycoprotein extracted from Anodonta woodiana) liposomes wereinvestigated. Multilamellar and large unilamellar vesicles of HB-Ⅰa liposomes were prepared by reverse-phase evaporationmethod, the diameters of products were mainly in 1～10mm. Keeping the cooling rate of about 20K/min was preferable. Thepreferential order of cryoprotectants was as follows: sucrose-glycine>sucrose>mannitol>PVP>glycine. The buffersolution used as a rehydration solution was better than pure water and sucrose solutions.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2004年第3期154-157,共4页
Chinese Journal of Pharmaceuticals
基金
国家自然科学基金重点项目(59836240)
国家自然科学基金项目(50376040)
上海市教委重点学科建设项目
上海市科委基金项目(03Dz19504)
关键词
糖蛋白
脂质体
冷冻干燥
海洋药物
glycoprotein
liposomes
freeze-drying
halobios medicine
