除虫菊愈伤组织的诱导和继代

Induction,preservation and differentiation of calliof Pyrethrum cinerariifolium Trev.

以除虫菊茎尖为外植体,MS培养基为基本培养基,对除虫菊愈伤组织的诱导和继代进行了系统研究。结果表明:外源激素2,4-D与KT的9种配比中,1.0与0.4mg/L的激素组合对除虫菊愈伤组织的诱导率可达88.0%;当蔗糖质量浓度为10～40g/L时,蔗糖质量浓度的大小与愈伤组织增长量成正比,与褐变开始时间呈负相关关系,当质量浓度为25g/L时,愈伤组织日平均生长量可达0.127g;适宜的光照时间可延长愈伤组织保存期并维持良好的组织结构,光照时间为4h/d时,愈伤组织褐化开始时间保持在27d左右。

With stem tip as explants and MS as basic culture medium,studies were carried out on continuing growth and induction of subculture of Pyrethrum cinerariifolium Trev..The results show that:among the nine different treatments with the combinations of 2,4-D and KT,the better one is 1.0 mg/L vs 0.4 g/L.By doing so,we can get an ideal induction rate 88.0%;when the concentration of sucrose is ranged from 10 to 40 g/L,it will be in the direct proportion with increment of callus,while with the inverse proportion with start time of callus browned.Especially when the concentration of sucrose is 25 g/L,the callus increment can get to 0.127 g/d;at the same time,proper illumination can prolong the preserving time and maintain an upper tissue structure.If it being illuminated by 4 h/d,the beginning time of being browned of callus will exceed 27 days.