具有维持造血干/祖细胞能力的新型豆类凝集素的分离、纯化及功能鉴定

Extraction, Purification and Function Analysis of a New Lectin Which Can Maintain The Multipotential of Hematopoietic Stem/progenitor Cells In vitro

一种分离自豆类的新型凝集素不仅具有凝集活性 ,还具有体外长期维持造血干 /祖细胞的能力 .由眉豆(Dolichoslablab)中分离得到了这种多亚基的凝集素———FRIL (Flt3receptor interactinglectin) ,并对它进行了核酸和蛋白质序列分析 .免疫细胞分析显示 ,它的受体是CD34+ 造血干 /祖细胞所特有的 .在培养基中添加这种凝集素可长期维持CD34+ 细胞存活和增殖能力 .以Flt3配基 (FL)作为对照 ,在 2 8天的培养时间内 ,相对于FL ,FRIL可维持细胞较高的G0 /G1期比例 (80 %以上G0 /G1期 )和长期培养中 (1 4天以上 ) 1 5倍以上的集落形成量 .可见FRIL通过滞留造血干 /祖细胞于G0 /G1期而维持它们的自我更新潜能 .

A new lectin extracted from lablab can maintain the multipotential of hematopoietic stem/progenitor cells in vitro for a long time in addition to its hemagglutination property. This new lectin FRIL (Flt3 rectptor interacting lectin) was purified from Dolichos lablab , its biological speciality was identified and its receptors were located on hematopoietic stem/progenitor cells. When CD34 + cells were cultured in the presence of this lectin or its counterpart FL, within the 28 days of culture, FRIL can keep more cells in G0/G1 phase (beyond 80%) and 1 5 fold colony forming cells more than FL does after 14 days culture. So, it is concluded that FRIL maintain HSC/HPCs self renewal property by halting its cell cycles.