大豆脂肪氧化酶-1缺失基因(lx_1)的RAPD标记

Detection of RAPD Makers Linked to Gene lx_1 in Soybean

采用RAPD技术,以大豆脂肪氧化酶缺失近等基因系Century(分别含有Lx、lx1、lx2、lx3、lx1.3、lx2.3基因)为材料,筛选了520个随机引物,发现13个引物在6个近等基因系材料中具有多态性,其中针对lx1、lx1.3基因的多态性引物有6个,分别为OPG061300、S352900、S370900、S377780、S287950、和S389300。引物S352经多次重复和组合96P11×Century-1的F2代分离群体检测,χ2测验符合1∶2∶1分离比率,表明S352与lx1基因紧密连锁,连锁距离为7.6 cM。

Near-isogenic lines of soybean lipoxygenase, which contain genes Lox, lx1, lx2, lx3, lx1.3, lx2.3, respectively, wereused for polymorphic analysis by RAPD technique. Five hundred and twenty 10-mer-oligonucleotide primers were screened,and thirteen primers showed polymorphism among near-isogenic lines. There were six primers showed special polymorphicbands in lines lx1 and lx1.3. Especially, primer S352 presented the stable results in which a 900 bp band was found in the lines lx1and lx1.3, and primer S352900 was detected with F2 generation of cross 96P11×Century-1, indicated primer S352900 could beidentified as a RAPD marker linked to gene lx1 in soybeans. The linkage distance was 7.6 cM.