水稻几丁质酶基因导入芥菜型油菜的研究

Studies on transgenic rapeseed(Brassica juncea)with rice chintinase gene by Agrobacterium tumefacien

以芥菜型油菜 (BrassicajunceaL .)下胚轴为转化材料 ,通过根癌农杆菌 (Agrobacteriumtumefaciens)介导将水稻的几丁质酶基因 (Ricechitinasegene)导入“泸洲四陵”油菜品种中 ,获得抗潮霉素的再生转基因植株 ,并讨论了影响油菜再生及转化效率的几个因素。对部分经潮霉素筛选得到的再生植株进行了多次重复PCR检测 ,发现其中 40 %以上的潮霉素抗性植株均表现出较强的阳性反应 ,初步证明几丁质酶基因已整合到油菜细胞核基因组中。

In an experiment with the hypocotyls of rapeseed ('luzhousiling'Brassica juncea L.) as explants in invitro culture, a transformation of introducing rice chitinase gene into'luzhousiling'by Agrobacterium tumefaciens was established. Some Hyg-resistant green shoots were obtained. PCR test of the resistant plants indicated that 40% of the Hyg-resistant plants showed strong positive reaction, suggesting that chitinase gene had been integrated into the genome of rapeseed.