Cr^(6+)、Cr^(3+)胁迫对黑藻生理生化影响的比较研究

Comparative studies of toxic effect of Cr^(6+),Cr^(3+) stress on the physiological and biochemical characteristics of Hydrilla verticillata

以沉水植物黑藻 (Hydrillaverticillata(L .f.)Royle)为实验材料 ,通过模拟水体Cr6+ 、Cr3 + 污染环境 ,比较研究了两种价态铬对黑藻叶的毒害影响。结果表明 :随着Cr6+ 、Cr3 + 浓度的加大 ,超氧阴离子 (O 2)产生速率、丙二醛 (MDA)、可溶性蛋白含量皆呈先升后降趋势。Cr6+ 、Cr3 + 浓度过高时 ,三种抗氧化酶 (SOD、POD、CAT)活性比例失衡 ,且Cr6+ 处理组的O 2 产生速率、MDA含量高于Cr3 + 处理组 ,叶绿素、可溶性蛋白含量、叶绿素a/b值低于Cr3 + 处理组 ,显示出Cr6+ 的毒性远大于Cr3 + 。

Toxic effects of Cr^(6+),Cr^(3+) on Hydrilla verticillata(L.f.)Royle cultured with simulated different concentration of Cr^(6+),Cr^(3+) water pollution were studied. The results showed that the O~~ 2 generation rate?chlorophyll and soluble protein content rose at first and fell subsequently with the increase of the two polluants. Three kinds of antioxidases disordered at higher concentration of Cr^(6+),Cr^(3+),O~~ 2 generation rate and MDA content of Cr^(6+) treatment were higher while chlorophyll and soluble protein content,ratio of chlorophyll a/b were lower than that of Cr^(3+) treatment. As a result,toxicity of Cr^(6+) to Hydrilla verticillata was more serious than that of Cr^(3+).