应用RAPD技术探讨红叶李、李和杏的亲缘关系

Genetic relationship of myrobalan plum,plum and apricot cultivars by RAPD analysis

为了对红叶李Prunuscerasiferacv.Atropurpurea,李P.salicina和杏P.armeniaca的亲缘关系、品种识别及资源保存提供分子生物学依据,也为了验证传统的形态学分类方法,从132个随机引物中筛选出20个引物对红叶李、李和杏的8个材料进行了基因组DNA扩增,均具有多态性,共扩增出264条带,平均每个引物产生13 2个RAPD片段。RAPD带型及聚类分析表明:RAPD技术能将红叶李、李属植物和杏属植物完全分开,红叶李、李和杏之间表现出一定的亲缘关系,各属品种之间都有不同的遗传距离,证明RAPD技术可作为种和属水平的分类鉴定依据;利用红叶李、李和杏品种的特异谱带结合DNA指纹,可将参试的各品种鉴别出来,从而说明了RAPD技术能够用于种或品种之间的鉴定。

.To provide evidence for conservation and identification of genetic relationship among myrobalan plum, plum and apricot cultivars and testify the traditional morphology-based taxonomy, genomic DNA's of 8 accessions are amplified by RAPD (random amplified polymorphic DNA) with 20 primers selected from 132 random primers. The result shows that they are all of polymorphism. 264 fragments had been amplified, with 13.2 RAPD fragments amplified from each primer on the average. Clustering analysis shows that myrobalan plum, plum and apricot cultivars can be distinguished by RAPD;there is some genetic relationship among them;and the distance between varieties of each genus is different. The genetic distance reflects genetic relationship among tested materials. It has been proved that RAPD technique can be used for the classification and identification of genera. We can identify each cultivar of tested materials by DNA fingerprints and the specific bands. It shows that RAPD can be used for the identification of species or cultivars.